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The AMMP assay uses a traditional assay format with proprietary detection technology providing accurate results, fewer sample limitations, simple operation and fast assay development.
Every aspect of the process — capture strategy, assay format, flow rate, reagent formulation, incubation time, etc. — is easily customizable to meet specific analytical needs. These flexible elements can be adjusted at run time to deliver different performance attributes based on research objectives. The universal surface found on the MEMS sensor allows for the investigation of one analyte or multiple analytes in the same experiment without the need for complex assay design or validated kits.
How does it work:
- Sample and calibrants are incubated with capture magnetic microparticles and tagged sandwiching antibodies in a microtiter plate.
- The mixture is then delivered to the anti-tag coated biosensor under timed continuous flow. The sensor is vibrating at its natural frequency. A magnetic field attracts the microparticles to the sensor surface.
- The magnet is released and only the biologically bound microparticles (with the analyte of interest) remain affixed to the sensor; un-bound beads are washed away.
- Buffer continues to flow over the sensors and the microparticles wash away in accordance with the strength of binding to the sensor surface, which is directly correlated to their analyte load. The bound microparticles cause a mass change on the vibrating membrane which creates a change in its frequency. As the microparticles wash off the surface, the system detects the frequency variation in a highly precise and quantitative way, and yields a dose-response curve.
- Regeneration buffer flows over the surface to prepare for the next assay.