Applications

BioScale’s novel AMMP platform enables ultra-sensitive picogram-level detection in complex matrices, making it suitable for a broad range of applications throughout research, life sciences, and diagnostics. Biomolecular detection at picogram/mL sensitivity levels using current methodologies is often hampered by labor-intensive protocols, inaccuracy and imprecision which affects productivity and costs.   BioScale has developed a powerful platform that provides ultra-sensitive solutions for improving data quality, workflow efficiency and productivity across a wide variety of analytes in complex matrices.

The platform has already demonstrated high-quality analytical performance in life sciences, biomarker discovery, and validation.  We are continuing to expand AMMP’s unique capabilities to deliver customer-focused solutions. Representative data exhibiting picogram-level detection in complex matrices using AMMP technology is shown in select applications:

• Protein-based detection in serum
• Ultra-Sensitive and Rapid Bacterial Detection
• Precision and reproducibility

Protein-Based Detection in Serum

The AMMP system offers ultra-sensitive and quantitative multiplexed protein measurement.  In this example, we detect and quantitate a protein (IL-6) in a serum sample. In figure 1, IL-6 serum samples are detected within the normal human range or biologically revelent levels using BioScale’s AMMP technology. In addition, BioScale’s AMMP is more sensitive than ELISA and a leading bead-based technology in a direct comparison using samples from the same matrix and capture antibodies.

In figure 2,  BioScale’s AMMP exhibits a lower limit of quantitation than ELISA.

Figure 1: LLOD comparison of IL-6 (pg/mL) in serum.		Figure 2: LLOQ of IL6 in serum.

Ultra-Sensitive and Rapid Bacterial Detection

Traditional culture-based systems are time-and labor-intensive. The AMMP system offers an ideal alternative for ultra-sensitive and rapid detection. AMMP-based assays can be much more rapid and sometimes even culture-free. 

Results for bacterial detection are often slow. Detecting enteric pathogens in stool typically takes two days for a culture-based method.  In the experiment below (Figure 3A and 3B) BioScale’s protocol takes only two hours for culture-free enteric pathogens detection in stool (E. coli and Salmonella).

Figure 3A) Detection of Salmonella in stool, two-hour protocol. Figure 3B) Detection of E. coli in stool, two-hour protocol.

Precision and Reproducibility

Life science markets seek solutions that deliver robust interrogation of biological systems rapidly and with high sensitivity, reproducibility at various levels of quantitation. BioScale’s AMMP system has been shown to outperform ELISAs with regard to ultra-sensitivity and precision using assay standards and customer-supplied samples.  As shown below, sensor-to-sensor repeatability range is <10% in assay-relevant concentrations, and instrument-to-instrument repeatability exhibited similar performance.

pg/mL IL6 AMMP RU SD %CV 0.63 0.120 0.016 13.4 1.25 0.225 0.018 8.1 2.50 0.382 0.023 6.1 5.00 0.571 0.030 5.2 10.00 0.735 0.031 4.2 20.00 0.824 0.030 3.7 40.00 0.852 0.043 5.0		pg/mL IL6 AMMP RU SD %CV N 0.625 0.135 0.012 9.1 8 1.25 0.241 0.015 6.4 8 2.5 0.400 0.024 6.1 8 5 0.581 0.025 4.3 8 10 0.755 0.023 3.0 8 20 0.836 0.020 2.4 8 40 0.894 0.019 2.1 8
Sample reproducibility data. Left: Data table for sensor-to-sensor variation. Right: Data table for instrument-to-instrument variation.